Simple Plex Human IL-6 3rd Gen Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-009384
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-6 3rd Gen Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 23.1 | 876 | 23.6 | 1008 |
| Standard Deviation | 0.899 | 72.1 | 1.82 | 77.7 |
| CV% | 3.9 | 8.2 | 7.7 | 7.7 |
Recovery for Simple Plex Human IL-6 3rd Gen Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 103-106 |
| EDTA Plasma (n=4) | 92 | 86-102 |
| Heparin Plasma (n=4) | 87 | 85-90 |
| Saliva (n=4) | 102 | 102-102 |
| Serum (n=4) | 94 | 90-99 |
| Urine (n=4) | 98 | 93-104 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-6 3rd Gen Cartridge
Human IL-6 (3rd gen) Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filters above to drill down to the IL-6 product that best fits your needs.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-6 Products
Product Documents for Simple Plex Human IL-6 3rd Gen Cartridge
Product Specific Notices for Simple Plex Human IL-6 3rd Gen Cartridge
For research use only